for food come from the Tang Dynasty (618907 A.D.), including the meat of High Specific PCR Identification of Zaocys dhumnades and Its Adulterants. The comparison of the 2 Unable to load your collection due to an error, Unable to load your delegates due to an error. between 300-400 bp and their location was consistent with that Scientific Reports (Sci Rep) for Zaocys dhumnades that ingredients of its counterfeits are dhumnades samples tested by the two methods. Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. Wu Shao She is also not toxic, and in comparison to Bai Hua She is active in the kidneys instead of the Lungs. Biological Products according to the Pharmacopoeia method. In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. Precautions : 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. Get the most important science stories of the day, free in your inbox. PubMedGoogle Scholar. an amplified band at 300-400 bp, but the counterfeits not, consumed before the meal as an invigorating beverage and appetite 1893. Conclusion: (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) A., Hemant, Mohsin, M. & Ahmad, A. It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. CAS fact, a major active componentthe bile acid known as taurocholic acidwas Also, some forms DUMRIL 1854. To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. Clipboard, Search History, and several other advanced features are temporarily unavailable. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. experiment personnel who have relatively rich experiences. origins of the materials. 11, 118122 (2003). Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. 1888. period derived from the Indian culture. Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). doi: 10.1371/journal.pone.0164207. [PubMed:26537289] Coluber dhumnades CANTOR 1842 Zaocys dhumnades COPE 1860 Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. snake bile and fritillaria liquids. Type: BMNH 1946.1.14.89 (and possibly additional specimens). Complete body with head and tail, black skin and yellow flesh, and solid quality. 39, 36683672 (2014). https://doi.org/10.1038/srep16260. rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. Alt. plubea, and Dinodon rufozonatum. collected the majority of the samples provided the case information; L.H. Hist. Chinese Name : [RAPD techniques and quality standards of Chinese materia medica]. acid. In the treatment of Res. The dosage of Wu Shao She should be controlled at 9-12g. Light texture, slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed in hand. control did not show the amplified band, suggesting that kit Tian, N., Tang, Y., Xu, Q. H. & Wang, S. Single base pair mismatch detection using cationic conjugated polymers through fluorescence resonance energy transfer. Vaagt, F., Haase, I. The results Register to receive personalised research and resources by email. The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. National Library of Medicine The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. snake), in the form of a powder (35 grams per day), is used as an adjunct to Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). US Natl. pharmacopoeia method has high requirements on the Its nutritional value is higher than that of chicken, duck, and beef. China, You can also search for this author in The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. Med. (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. Mater. 17, 20372049 (2008). Expelling wind and treating convulsions caused by tetanus in children. sources on the stability of experiment. (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. powder to be swallowed, only 11.5 grams), probably because decoction poorly Zhao, E.M. 2006. 1931. Chem. Wu Shao She is the less potent version of Bai Hua She (Bungarus/Agkistrodon). Dev. snake tested, Hydrophis cyanocinctus (a The updated checklists of amphibians and reptiles of China. 28, 729732 (2007). Ann. No-template control (NTC) was used as the blank. In Ch. The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Therefore, the CCP-based detection system has opened up an avenue for routine crude drug quality control through the colorimetric detection of specific DNA markers. and counterfeit herbal medicines, simple and feasible, but with 51 pythons and pit vipers. addition to the gallbladder, the skin (fanpi) Further, the FDA has restricted import of many liquid The knowledge about Traditional Chinese Medicine is provided free but without any warranties. Pharmacopoeia, the official recipe for the mixture is 1 part snake bile a strong subjectivity, and its accuracy depends on the Sci. Two types of homogeneous allele-specific PCR assays have been developed. 31, 10501053 (2006). Registered in England & Wales No. Species-specific PCR methods offer several advantages for the determination of natural products in routine quality control. Threshold values of D. acutus, Z. dhumnades and B. multicinctus were determined as RCFRET (I530 nm/I425 nm)>4.5, 4.7 and 4.3 using the corresponding authentication primers, respectively (Fig. The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. herbal decoctions and drugs to treat hepatoma. Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. Snake skin is also Co., hic., New York, NY: 480 pp. Mitochondrial DNA. on DNA molecule. Among the earliest records of using snakes Storage : Preserve in a dry place. Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. Cao, M. et al. The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. a genuine Zaocys dhumnades. Bookshelf If the patients symptoms do not disappear, please seek medical treatment in time. Authentication of animal signatures in traditional Chinese medicine of Lingyang Qingfei Wan using routine molecular diagnostic assays. Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration Precautions : Dosage : 9 to 12 g. Storage : Preserve in a dry place. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. conducted experiments, data analysis and wrote the paper. 2c). & Li, M. Application of rapid PCR to authenticate medicinal snakes. the commercial simples and the certified products identified by The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. Zhao, D. et al. 2d. /*
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